Coding

Part:BBa_K3039006:Design

Designed by: Ceilidh Welsh   Group: iGEM19_Exeter   (2019-08-29)


BHETase 2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1104
    Illegal PstI site found at 653
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1104
    Illegal PstI site found at 653
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1104
    Illegal BamHI site found at 561
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1104
    Illegal PstI site found at 653
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1104
    Illegal PstI site found at 653
    Illegal NgoMIV site found at 246
    Illegal AgeI site found at 1130
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The amino acid sequence was submitted to Twist Bioscience who codon optimised the sequence for E. coli, ensuring that there were no forbidden restriction sites, BsaI or SapI, to allow for potential TypeIIS assembly.


Source

Wild type amino acid sequence originates from Ideonella sakaiensis. Mutations have been directed by reference to the literature. Resulting DNA sequences were codon optimized by TWIST biosciences.


References